Table 2.
Related gene and primer information.1
| Genes1 | Orientation | Primer sequences (5′-3′) | Product size | Accession number |
|---|---|---|---|---|
| β-actin | Forward | GCTACAGCTTCACCACCACA | 90 | NM_205518.1 |
| Reverse | TCTCCTGCTCGAAATCCAGT | |||
| IL-1β | Forward | ACTGGGCATCAAGGGCTA | 117 | NM_205518.9 |
| Reverse | GGTAGAAGATGAAGCGGGTC | |||
| IL-6 | Forward | CAGGACGAGATGTGCAAGAA | 98 | NM_205518.10 |
| Reverse | GAGAGCTTCGTCAGGCATTT | |||
| TNF-α | Forward | AGATGGGAAGGGAATGAACC | 139 | XM_040647309.1 |
| Reverse | GGAAGGGCAACTCATCTGAA | |||
| NF-κB | Forward | GCTGCTTTGCACAGATGGA | 130 | NM_205134.1 |
| Reverse | CCTTTGCAAAACTGGTTGGT | |||
| Claudin-1 | Forward | GTCTTTGGTGGCGTGATCTT | 117 | NM_001013611.2 |
| Reverse | TCTGGTGTTAACGGGGTGTGA | |||
| Claudin-2 | Forward | CTTTGCTTCATCCCACTGGT | 82 | NM_001277622.1 |
| Reverse | TCAAATTTGGTGCTGTCAGG | |||
| Mucin-1 | Forward | CCGTGGTGAAGAGGATTTGT | 126 | XM_015279045.2 |
| Reverse | TTGGATGCAGTCACACCATT | |||
| Mucin-2 | Forward | ACCAAGCAGAAAAGCTGGAA | 80 | NM_001318434.1 |
| Reverse | AAATGGGCCCTCTGAGTTTT | |||
| ZO-1 | Forward | GGCAAGTTGAAGATGGTGGT | 130 | XM_01527898.2 |
| Reverse | ATGCCAGCGACTGAATTTCT | |||
| StAR | Forward | AGAGGAAGCCCTGCAGAAAT | 92 | NM_204686.2 |
| Reverse | TCAGCACTTTGTCTCCGTTG | |||
| AR | Forward | CCTGAATGAACTTGGGGAGA | 93 | NM_001040090.1 |
| Reverse | ATCTGGTCATCCACATGCAA | |||
| ESR1 | Forward | TTCAAGGGGAGGAATTTGTG | 123 | NM_205138.2 |
| Reverse | TGTCCAGAACACGGTGGATA | |||
| ACVR1 | Forward | ATTCCCAGAGCACAAACCAG | 93 | NM_001110204.1 |
| Reverse | GGATGGTTTCATCGAGCACT |
1
Abbreviations: ACVR1, activin a receptor type 1; AR, androgen receptor; ESR1, estrogen receptor 1; IL-1β, interleukin-1β; IL-6, interleukin-6; StAR, steroidogenic acute regulatory protein; TNF-α, tumor necrosis factor-α; ZO-1, zonula occluden-1.