Fig. 2.

Mechanisms other than cell apoptosis involved in fisetin-induced OC cell death. (A) Cytometric results of annexin V and propidium iodide (AV/PI) staining of A2780 cells. (B) Quantification of apoptotic A2780 cells treated with fisetin ± z-VAD. (C) Relative expression level of Cyt-C translated in the mitochondria of A2780 cells treated with or without fisetin (100 mmol/L) for 72 h. (D) Comparison of fisetin-treated A2780 cell growth in a time-dependent manner, revealing that the inhibition of apoptosis by z-VAD failed to promote cell growth after fisetin treatment. (E–H) Identical experiments and intervention were performed for the other OC cell line, OVCAR-3. *, p < 0.05