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Cell necroptotic pathways regulate fisetin-induced cell death. (A) In A2780 cells, the expression of HMGB1, ZBP1, RIP3 and MLKL in the control and fisetin + z-VAD treatment groups was measured by Western blot. (B) Quantification and comparison of the levels of HMGB1, ZBP1, RIP3 and MLKL expression. (C, D) Western blot results and quantification of HMGB1, ZBP1, RIP3 and MLKL in OVCAR-3 cells under the respective interventions. *, p < 0.05
