Fig. 5.

ZBP1 knockdown increased the migration of OVCAR-3 cells, blocking fisetin-induced apoptosis. A Western blotting of both the ZBP1 knockdown (ZBP1^−/−) group and the control group, in which OVCAR-3 cells were treated with fisetin + z-VAD. (B) Quantification of ZBP1 expression with or without ZBP siRNA administration. (C) In vitro invasion assay between the respective groups. (D) MTT assay indicated differences in cell migration between the indicated groups. (E) Western blotting and (F) quantitative expression levels targeting RIP3 and MLKL in both ZBP1^−/− and normal OVCAR-3 cells, both of which were treated with fisetin + z-VAD. *, p < 0.05