Fig. 1.

CAFs acquisition methods. Fibroblasts were cultured in the presence of melanoma cells cultured on Transwell inserts (INS) or with a melanoma-conditioned cell culture medium (CM) for 7 days. Next, cells were harvested and used for further experiments (analysis of migration, invasion, proliferation, level of intracellular proteins involved in cancer progression), or medium was changed for FBM without FBS, collected after 72 h and then used for the analysis of extracellular proteins level