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. 2022 May 10;20:63. doi: 10.1186/s12964-022-00871-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Identification of CAFs. Western Blot analysis of protein level of FAP (A) in cell lysates and IL6 (B) in media collected from activated fibroblasts cultured with melanoma-conditioned media (CM) or with melanoma cells growing on Transwell inserts (INS). Signal was normalized to total protein content assessed by Ponceau S staining. Control (CTRL) constitutes fibroblasts cultured in FBM:DMEM (1:1 ratio) media analogous to tested cells. In the case of IL6, due to the lack of signal in CTRL, the fold is set vs signal from the last lane (Hs294T). The mean of at least three biological repetitions ± SD is shown. Asterisks indicate statistically important differences between control cells and CAFs or between different types of CAFs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), and p ≤ 0.0001 (****)