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. 2022 May 10;20:63. doi: 10.1186/s12964-022-00871-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Analysis of CAFs’ migratory and invasive abilities obtained in culture with melanoma-conditioned media (CM) or melanoma cells on Transwell inserts (INS). Control (CTRL) constitutes of fibroblasts cultured in FBM:DMEM (1:1 ratio) analogous to tested cells. To investigate migration and invasion A 2D and B 3D wound healing assays were performed. Relative wound density—determined using IncuCyte® Live-Cell Analysis System—is shown. Transwell invasion tests were also conducted (C). The number of control cells that invaded was set as 100%. The relative invasion factor was calculated. All experiments were conducted three times, each performed in triplicate and the mean ± SD is shown. Asterisks indicate statistically important differences between control cells and CAFs or between different types of CAFs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), and p ≤ 0.0001 (****)