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. 2022 May 10;20:63. doi: 10.1186/s12964-022-00871-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Matrix metalloproteases’ activity in CAFs. A Images of representative gelatin zymography gels performed on media collected from CAFs. The mean of densitometry quantification of at least three independent repetitions of gelatin zymography ± SD is shown. B As MMP14 is a membrane metalloprotease, its activity was measured in cell lysates collected from CAFs, using a fluorimetric activity assay. The data represent the mean MMP14 activity of three independent measurements ± SD. In both experiments, CAFs were obtained from fibroblasts cultured with melanoma-conditioned media (CM) or with melanoma cells on Transwell inserts (INS). Control (CTRL) constitutes of samples collected from fibroblasts cultured in a composition of FBM:DMEM (1:1 rate) medium. Asterisks indicate differences between control cells and CAFs or between different types of CAFs. The significance level was set at p ≤ 0.05 (*), p ≤ 0.01 (**) and p ≤ 0.001 (***)