KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| human GPC2 Clone D3, Dylight650 labelled) | Dimiter S. Dimitrov (university of Pittsburgh) | N/A |
| human GPC2 Clone 19, Dylight650 labelled) | This manuscript | N/A |
| human isotype CTRL (Dylight650 labelled) | Crown Bioscience | Cat# C0001 |
| human CD4-BUV395 (Clone SK3) | BD Biosciences | Cat# 563550 |
| human CD8-BUV805 (clone SK1) | BD Biosciences | Cat# 564912 |
| human PD1-PE-Cy7 | Thermo Fisher Scientific (ebioscience) | Cat# 25–2799-41 |
| human Tim3-BV510 (Clone F38–2E2) | Biolegend | Cat# 345030 |
| human Lag3-PE | Thermo Fisher Scientific (ebioscience) | Cat# 12–2239-42 |
| human CD62L-BV650 (Clone DREG-56) | BD Biosciences | Cat# 562719 |
| human CD45RA-FITC (Clone L48) | BD Biosciences | Cat# 649458 |
| FMC63-anti-idiotype (Dylight650 labelled) | Dr. Laurence Cooper, MD Anderson Cancer Center | N/A |
| human CD13-BUV737 (L138) | BD Biosciences | Cat# 749265 |
| human CD45-PerCp-Cy5.5 | Thermo Fisher Scientific | Cat# 45–0459-42 |
| human GPC2 (Clone D3, PE labelled) | Dr. Dimiter S. Dimitrov (university of Pittsburgh) | N/A |
| human GD2-BV510 (Clone 14g2a) | Biolegend | Cat# 357316 |
| human NCAM-BV605 (Clone 5.1H11) | Biolegend | Cat# 362538 |
| human L1CAM-BV421 (Clone 5G3) | BD Biosciences | Cat# 565732 |
| human ALK (Clone 48, APC labelled) | Mark Vigny | N/A |
| human B7-H3-PE-Cy7 (Clone MIH42) | Biolegend | Cat# 351008 |
| human GD2-PE-Cy7 (Clone 14g2a) | Biolegend | Cat# 357308 |
| human NCAM-BV510 (Clone 5.1H11) | Biolegend | Cat# 362534 |
| human L1CAM-BUV395 (Clone 5G3) | BD Biosciences | Cat# 565736 |
| human B7-H3-BUV805 (Clone 7–517) | BD Biosciences | Cat# 748378 |
| human HVCN1-FITC (polyclonal) | LS BIO | Cat# LS‑C236467 |
| Isotype CTRL FITC (polyclonal) | LS BIO | Cat# LS-C149376 |
| human CD3-BUV737 (Clone UCHT1) | BD Biosciences | Cat# 612750 |
| human CD19-BUV495 (Clone SJ25C1) | BD Biosciences | Cat# 612938 |
| human CD15-BV711 (Clone W6D3) | Biolegend | Cat# 323050 |
| human CD66b-PE-Cy7 (Clone G10F5) | Biolegend | Cat# 305116 |
| murine CD45-PacBlue (Clone ) | Biolegend | Cat# 103126 |
| murine TER-119-PacBlue (Clone ) | Biolegend | Cat# 116232 |
| murine H-2Kb-PacBlue (Clone ) | Biolegend | Cat# 116514 |
| murine CD31-PacBlue (Clone ) | Biolegend | Cat# 102422 |
| Bacterial and Virus Strains | ||
| Stellar Competent Cells | Clontech, Takara | Cat# 636766 |
| Biological Samples | ||
| Buffy Coats | Stanford Blood Bank | N/A |
| Whole Blood Samples of healthy donors | Stanford Blood Bank | N/A |
| Human Neuroblastoma Primary Tumor Microarray (TMA) | Children’s Oncology Group (COG) | N/A |
| Tissue Blocks of human prenatal and infant brain | as described in Theruvath et. al, Nature Medicine 2020 | N/A |
| Tissue Blocks of human pediatric brain | BC Children’s Hospital Vancouver | N/A |
| Human Neuroblastoma infiltrated Bone Marrow Samples | Stanford Pediatric Bass Center Tissue bank (IRB: #45458 and #56619) | N/A |
| Human Neuroblastoma infiltrated Bone Marrow Samples | Children’s Cancer Research Institute; Vienna Austria (EK#115/2006, EK#1853/2016 and EK#1216/2018) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Phusion Hot Start Flex 2X Master Mix | New England Biolabs | Cat# M0536L |
| Rapid DNA Ligation Kit | Roche | Cat# 11–635-379–001 |
| In-fusion HD Cloning System | Clontech, Takara | Cat# 639647 |
| ZymoPURE II Plasmid Maxiprep Kit | Zymo Research | Cat# D4203 |
| RosetteSep Human T cell Enrichment kit | Stem Cell Technologies | Cat# 15061 |
| Dynabeads Human T-Expander CD3/CD28 | Thermo Fischer Scientific, Gibco | Cat# 11141D |
| Lipofectamine 2000 Transfection Reagent | Thermo Fischer Scientific | Cat# 11668500 |
| RetroNectin® Recombinant Human Fibronectin Fragment | Takara | Cat# T100B |
| Recombinant Human IL-2 | Preprotech | Cat# 200–02-1mg |
| Recombinant Human Glypican 2 Protein | R&D systems, Inc. | Cat# 2304-GP-050 |
| Recombinant Mouse Glypican 2 Protein | R&D systems, Inc. | Cat# 2355-GP-050 |
| Dylight 650 Micoscale Antibody Labelling Kit | Thermo Fischer Scientific | Cat# 84536 |
| Dylight 488 Micoscale Antibody Labelling Kit | Thermo Fischer Scientific | Cat# 53025 |
| Abcam PE Conjugation kit Lightning-Link | Abcam | Cat# ab102918 |
| Abcam APC Conjugation kit Lightning-Link | Abcam | Cat# ab201807 |
| BD QuantiBRITE PE Beads | BD Biosciences | Cat# 340495 |
| BD Custom Quantitation Beads in PE-Cy7, APC, BV421, BV510, BV605 | BD Biosciences | N/A |
| Critical Commercial Assays | ||
| Human IFN-γ ELISA MAX Deluxe | BioLegend | Cat# 430104 |
| Human IL-2 ELISA MAX Deluxe | BioLegend | Cat# 431804 |
| Whole Blood Column Kit | Miltenyi | Cat# 130–093-545 |
| StraightFrom Whole Blood CD19 MicroBeads, human | Miltenyi | Cat# 130–090-880 |
| StraightFrom Whole Blood CD66b MicroBeads, human | Miltenyi | Cat# 130–104-913 |
| Deposited Data | ||
| scRNAseq data human and murine prenatal brain | La Manno et. al, 2016 | GEO (accession GSE76381) |
| scRNAseq data human adult brain | Allen Brain Atlas | https://portal.brain-map.org/atlases-and-data/rnaseq/human-multiple-cortical-areas-smart-seq |
| Experimental Models: Cell Lines and PDX | ||
| CHO (female) | ATCC | RRID:CVCL_0213 |
| HS-5 (male) | ATCC | RRID:CVCL_3720 |
| NGP (male) | Dr. John Maris (CHOP) | RRID:CVCL_2141 |
| NGP-NPC2 (male) | Dr. John Maris (CHOP) | N/A |
| SMS-SAN (female) | Dr. John Maris (CHOP) | RRID:CVCL_7136 |
| NBSD (female) | Dr. John Maris (CHOP) | RRID:CVCL_LF68 |
| EBC1 (male) | Dr. John Maris (CHOP) | RRID:CVCL_E218 |
| NB1643 (male) | Dr. John Maris (CHOP) | RRID:CVCL_5627 |
| CHLA255 (sex unspecified) | Dr. Robert Seeger (Keck School of Medicine, USC) | RRID:CVCL_AQ27 |
| SMS-KCNR (male) | Dr. Robert Seeger (Keck School of Medicine, USC) | To cite this cell line use: SMS-KCNR (RRID:CVCL_7134 |
| SKNAS (female) | Dr. Bill Chiu (Stanford University) | RRID:CVCL_1700 |
| CHLA-90 (male) | Dr. Bill Chiu (Stanford University) | RRID:CVCL_6610 |
| Kelly (female) | Dr. Kimberly Stegmaier (Harvard Medical School) | RRID:CVCL_2092 |
| Lan5 (male) | Dr. Kimberly Stegmaier (Harvard Medical School) | RRID:CVCL_0389 |
| ST16-BM4045 (male, diagnostic) | This manuscript | N/A |
| ST16-BM4224 (male, relapse) | This manuscript | N/A |
| COG-N-421x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-426x-Felix PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-440x PDX (female) | Dr. John Maris (CHOP) | N/A |
| COG-N-453x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-471x PDX (female) | Dr. John Maris (CHOP) | N/A |
| COG-N-496x PDX (female) | Dr. John Maris (CHOP) | N/A |
| COG-N-519x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-549x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-557x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-561x PDX (female) | Dr. John Maris (CHOP) | N/A |
| COG-N-590x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-603x PDX (male) | Dr. John Maris (CHOP) | N/A |
| COG-N-624x PDX (female) | Dr. John Maris (CHOP) | N/A |
| CHLA-79 PDX (female) | Dr. John Maris (CHOP) | N/A |
| NB-1643 PDX (male) | Dr. John Maris (CHOP) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mice: NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) | The Jackson Laboratory | Cat# JAX:005557; RRID:IMSR_JAX:005557 |
| Oligonucleotides | ||
| N/A | ||
| Recombinant DNA | ||
| RD114 | add | add |
| CAR.GPC2.D3VLVH.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.D3VHVL.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.D4VLVH.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.D4VHVL.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.19VLVH.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.19VHVL.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.27VLVH.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.27VHVL.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.CH2CH3.D3VLVH.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.CH2CH3.19VLVH.8aHTM.BBz | This manuscript | N/A |
| CAR.GPC2.D3VLVH.28HTM.BBz | This manuscript | N/A |
| CAR.GPC2.19VLVH.28HTM.BBz | This manuscript | N/A |
| CAR.GPC2.D3VLVH.28HTM.28z | This manuscript | N/A |
| CAR.GPC2.19VLVH.28HTM.28z | This manuscript | N/A |
| CAR.cJUN-P2A-GPC2.19VLVH.28HTM.28z | This manuscript | N/A |
| CAR.CD19.FMC63.8aHTM.BBz | previously used in our lab | N/A |
| Software and Algorithms | ||
| FlowJo v10.7.1 | FlowJo, LLC | N/A |
| GraphPad Prism v8.4 | GraphPad Software Inc. | N/A |
| Living Image version (IVIS imaging) | Perkin Elmer | N/A |
| Biorender | Biorender | N/A |
| Scanpy v 1.6.0 (ssRNAseq analysis) | Wolf et al., 2018 | N/A |
| Other | ||
| fixable viability dye eFluor780 | Thermo Fisher Scientific (ebioscience) | Cat# 65–0865-14 |
| CountBright Absolute Counting beads | Thermo Fischer Scientific | Cat# C36950 |
| Brilliant Stain buffer | BD Biosciences | Cat# 566349 |
| Human FC Block | BD Biosciences | Cat# 564219 |
| Corning CellStripper Dissociation Reagent | Corning | Cat# MT25056CI |
Add info for ssRNAseq datasets