Table 3.

Effect of ulvan lyase (ID 172) on proteins, pigments and fatty acid content (mg/g alga) of supernatant and residue¹.

Item	Supernatant				Residue
	Control2	UL3	SEM	P-value	Control2	UL3	SEM	P-value
Total proteins	36.7	38.0	4.68	0.861	148.0	164.1	11.26	0.369
Chlorophyll a	0.0194	0.013	0.0030	0.187	0.6085	0.636	0.0128	0.198
Chlorophyll b	0.0244	0.018	0.0057	0.474	0.4845	0.521	0.0145	0.145
Total chlorophylls	0.0434	0.031	0.0086	0.357	1.0925	1.157	0.0267	0.160
Total carotenoids	0.0114	0.008	0.0008	0.053	0.1175	0.115	0.0037	0.766
Total chlorophylls + Total carotenoids	0.0544	0.038	0.0091	0.300	1.2095	1.272	0.0277	0.180
Total pheophytins	0.1984	0.143	0.040	0.386	5.0925	5.222	0.1624	0.602

¹Mean values are based on 3 replicates per treatment.

²Control, 2 mL of resuspended macroalgae (20 g/L solution of PBS).

³UL, ulvan lyase treatment; 2 mL of macroalgae suspension at 20 g/L plus ulvan lyase (ID 172) at 20 mg/L in PBS solution.

⁴Values measured in PBS.

⁵Values measured after extraction with acetone.