Fig. 5.

Integrin αV is less abundant in a human basal-like breast cancer line in comparison to luminal breast cancer or non-tumoral cells. A) Western blot showing the relative amount of integrin αV in sub confluent monolayer cultures of MCF-10A, MCF-7 and MDA-MB231 cell lines. The basal-like cancer cell line MDA-MB231 expresses 50% less integrin αV than the non-tumoral cell line MCF1-0A. The luminal breast cancer cell line MCF-7 still presented high levels of integrin αV. B) ITGAV mRNA expression analysis in breast cell lines using the online tool Genevestigator revealed a decrease in ITGAV levels for both tumoral cell lines (MCF-7 and MDA-MB231). C) Immunofluorescence of integrin αV (green) in breast cell lines grown as monolayers. Integrin αV fluorescence staining was weaker in the membrane of MDA-MB231 cells. DAPI (blue) was used to stain nuclear DNA. Scale bar = 5 µm.