TABLE 1.
Mean specific activities of enzymes in crude extracts of glucose-grown strain VeGlc2 cellsa
| Enzyme activity | EC no. | Sp act (μmol per mg of protein per min) |
|---|---|---|
| Hexokinase (ATP) | 2.7.1.1/2 | 0.036 (0.004)b |
| Hexokinase (PEP) | <0.001 | |
| Hexokinase (P2O54−) | <0.001 | |
| Glucose-6-phosphate 1-dehydrogenase (NADP) | 1.1.1.49 | 0.019 (0.003) |
| Phosphogluconate 2-dehydrogenase (NAD) | 1.1.1.43 | 0.012 (0.001) |
| Phosphogluconate 2-dehydrogenase (NADP) | 1.1.1.43 | 0.014 (0.001) |
| Glucose-6-phosphate isomerase | 5.3.1.9 | 0.188 (0.009) |
| 6-Phosphofructokinase (ATP) | 2.7.1.11 | <0.001 |
| 6-Phosphofructokinase (P2O54−) | 2.7.1.90 | 1.93 (0.21) |
| Fructose bisphosphate aldolase | 4.1.2.13 | 0.048 (0.018) |
| Triose phosphate isomerase | 5.3.1.1 | 47.9 (9.5) |
| Glyceraldehyde-3-phosphate dehydrogenase | 1.2.1.12 | 1.48 (0.24) |
| 3-Phosphoglycerate kinase | 2.7.2.3 | 0.778 (0.065) |
| Enolase | 4.2.1.11 | 2.46 (0.04) |
| Phosphoglycerate mutase | 5.4.2.1 | 0.071 (0.005) |
| Pyruvate kinase | 2.7.1.40 | 0.037 (0.004) |
| Pyruvate synthase | 1.2.7.1 | 0.026 (0.003) |
| Malate dehydrogenase (NAD) | 1.1.1.37 | 28.5 (2.3) |
| Fumarate hydratase | 4.2.1.2 | 5.56 (0.82) |
| Succinate dehydrogenase | 1.3.99.1 | 0.045 (0.024) |
| Fumarate reductase | 1.3.99.1 | 0.019 (0.007) |
| Succinate-CoA ligase | 6.2.1.4/5 | 0.112 (0.013) |
The specific activities of the following enzymes were less than 0.001 μmol per mg of protein per min: glucose 1-dehydrogenase (NAD) (EC 1.1.1.118), glucose 1-dehydrogenase (NADP) (EC 1.1.1.119), glucose-6-phosphate 1-dehydrogenase (NAD) (EC 1.1.1.49), malate dehydrogenase (NADP) (EC 1.1.1.82), pyruvate orthophosphate dikinase (EC 2.7.9.1), pyruvate H2O dikinase (EC 2.7.9.2), acetate kinase (ATP) (EC 2.7.2.1), acetate kinase (P2O54−) (EC 2.7.2.12), propionate kinase (ATP) (EC 2.7.2.-), propionate kinase (P2O54−) (EC 2.7.2.-), pyruvate decarboxylase (EC 4.1.1.1), methylmalonyl-CoA:pyruvate transcarboxylase (EC 2.1.3.1), PEP carboxylase (EC 4.1.1.31), PEP carboxykinase (EC 4.1.1.32), pyruvate carboxylase (ATP) (EC 6.4.1.1), pyruvate carboxylase (GTP) (EC 6.4.1.-), methylmalonyl-CoA decarboxylase (EC 4.1.1.41), succinate:propionyl-CoA CoA-transferase (EC 2.8.3.-), succinate:acetyl-CoA CoA-transferase (EC 2.8.3.-), acetate-CoA ligase (EC 6.2.1.1/13), propionate-CoA ligase (EC 6.2.1.17), phosphate acetyltransferase (EC 2.3.1.8), and phosphate propionyltransferase (EC 2.3.1.-).
The values in parentheses are standard deviations, based on three to six determinations.