| Antibodies |
|
|
|
| Pan-flavivirus envelope protein-specific monoclonal antibody 4G2 Absolute Antibody (clone D1-4G2-4-15) |
Absolute Antibody |
Cat#Ab00230-2.0; RRID: AB_2715504 |
| Peroxidase conjugated affini-pure goat anti-mouse IgG Fc γ |
Jackson ImmunoResearch |
Cat. #115-035-008; RRID: AB_2313585 |
|
| Bacterial and virus strains |
|
|
|
| ZIKV FSS13025 (Cambodia, 2010) |
WRCEVA, (Galveston, TX) |
N/A |
| DENV2 (S221) |
(Yauch et al., 2009) |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
|
|
| Carboxymethyl cellulose |
Sigma-Aldrich |
Cat#9004-32-4 |
| TrueBlue peroxidase substrate |
KPL |
Cat#5510-0030 |
| ZIKV E protein |
The Native Antigen |
N/A |
| Qiamp Viral Mini Kit |
Qiagen |
Cat. #52904 |
| RNAlater |
Thermo Fisher Scientific |
Cat. #AM7021 |
| QuantaBio qScript one-step qRT-PCR kit |
VWR |
Cat. #101414-172 |
| StemProTM AccutaseTM Cell Dissociation Reagent |
Thermo Fisher Scientific |
Cat. #A1110501 |
|
| Experimental models: Cell lines |
|
|
|
| Baby hamster kidney (BHK)-21 cells |
ATCC |
Cat. #ATCC: CCL-10 RRID: CVCL_1915 |
| Aedes albopictcus: C6/36 cells |
ATCC |
Cat. #ATCC: CRL-1660 RRID: CVCL_Z230 |
| Vero cells |
ATCC |
(CRL-1586) |
| Human fetal neural progenitor cells (NPCs) |
A. V. Terskikh |
N/A |
|
| Experimental models: Organisms/strains |
|
|
|
|
Ifnar1−/− mice (C57BL/6 background) (males and females) |
(Yauch et al., 2009) |
N/A |
| BALB/c mice (males) |
Jackson Labs |
Cat. #000651 |
| Aedes aegypti mosquitoes (Rockefeller strain) |
P.-Y. Shi |
N/A |
|
| Oligonucleotides |
|
|
|
| Primers used for construction of recombinant ZIKV viruses |
Table S1
|
N/A |
| Primers and probes used for determination of ZIKV viral burden |
Table S2
|
N/A |
|
| Other |
|
|
|
| KPL True Blue |
SeraCare |
Cat. #5510-0030 |
| Leibovitz’s L-15 |
Thermo Fisher Scientific |
Cat. #11415064 |
| Fetal bovine serum (FBS) |
Thermo Fisher Scientific |
Cat. #16000044 |
| Penicillin/streptomycin |
Thermo Fisher Scientific |
Cat. #15140122 |
| HEPES |
Thermo Fisher Scientific |
Cat. #15630080 |
| High-glucose Dulbecco’s modified Eagle’s medium |
Thermo Fisher Scientific |
Cat. #11995065 |
| DMEM/F12 |
Corning |
Cat. #10-092-CV |
| N-2 supplement |
Thermo Fisher Scientific |
Cat. #17502048 |
| B-27™ supplement (50X), minus vitamin A |
Thermo Fisher Scientific |
Cat. #12587010 |
| human FGF-basic |
Thermo Fisher Scientific |
Cat. #PHG0263 |
| MEM non-essential amino acids solution |
Thermo Fisher Scientific |
Cat. #11140050 |
| Formalin |
Fisher |
Cat. #SF98-4 |
| Triton X-100 |
Sigma |
Cat. #X100-100ML |
| RNeasy Mini Kit |
Qiagen |
Cat. #73404 |
| AvrII |
(New England BioLabs) |
Cat. #R0174S |
| SphI |
(New England BioLabs) |
Cat. #R0182S |
| NaeI |
(New England BioLabs) |
Cat. #R0190S |
| ClaI |
(New England BioLabs) |
Cat. #R0197S |
| mMESSAGE mMACHINE™ T7 transcription kit |
(Thermo Fisher Scientific) |
Cat. #AM1344 |
| Complement-inactivated sheep blood |
(Hemostat laboratories) |
Cat. #C26218
|
