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. 2022 May 9;221(7):e202109013. doi: 10.1083/jcb.202109013

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© 2022 Cail et al.

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Figure 3. — Induced curvature does not rescue nucleation disruption. (A and B) Representative images of cells from flat substrate (A) or 75-nm ridges (B) expressing CLTA RFP or dynamin2 GFP with control or AP2 siRNA. Scale bar, 10 μm. (C) Quantification of CLTA or dynamin2 puncta density as a function of induced curvature; **, P < 0.01; *, P < 0.05; ns, P > 0.05 from multiple t test with Welch correction. Mean ± SD, n = 3 cells per condition. (D) Enrichment score for clathrin and dynamin is reduced after AP2 knockdown. **, P < 0.01; *, P < 0.05; ns, P > 0.05 from multiple t test. Mean ± SD, n = 3 cells per condition. (E) Model of endocytic protein localization after AP2 knockdown, showing no detectable difference without nucleating factor AP2.