TABLE 3.
NAI IC50 values for mAbs against all EMVsa
| Virus/rNA | Inhibitory activity (μg/mL) (95% confidence interval) |
||||||
|---|---|---|---|---|---|---|---|
| EM-2E01 | 1000-1D05 | 1000-3B04 | 1000-3B06 | 294-16-009-A-1C02 | 300-16-005-G-2A04 | Oseltamivir | |
| Irrelevant IgG control virus B | 0.012 (0.011–0.014) | 0.076 (0.059–0.098) | 0.54 (0.48–0.62) | 0.15 (0.13–0.18) | 5.65 (4.61–6.99) | 0.059 (0.052–0.066) | 0.087 (0.073–0.092) |
| N88D EMV | 0.018 (0.015–0.021) | 0.078 (0.60–0.10) | 0.70 (0.56–0.86) | 0.23 (0.20–0.26) | 12.03 (7.76–22.18) | 0.067 (0.057–0.079) | 0.051 (0.046–0.057) |
| N270D EMV | 0.014 (0.011–0.016) | 0.50 (0.29–0.90) | >30* | 11.25 (8.68–15.21) | >30* | 2.97 (2.21–4.10) | 0.088 (0.069–0.11) |
| Q313K EMV | 0.032 (0.030–0.035) | 8.50 (6.61–11.21) | 1.66 (1.40–1.98) | 0.45 (0.37–0.55) | >30* | 0.16 (0.14–0.19) | 0.15 (0.13–0.18) |
| Q313R EMV | 0.015 (0.013–0.017) | 0.11 (0.074–0.18) | 0.77 (0.67–0.89) | 0.19 (0.16–0.26) | >30* | 0.066 (0.052–0.082) | 0.070 (0.062–0.080) |
| S364N EMV | >30* | 0.90 (0.62–1.31) | 1.02 (0.78–1.34) | 0.32 (0.23–0.44) | 11.77 (9.34–15.40) | 0.13 (0.097–0.16) | 1.259 (0.87–1.86) |
| S364N/N369T EMV | >30* | 1.61 (1.00–2.67) | 2.04 (1.61–2.61) | 0.29 (0.21–0.41) | 8.05 (5.69–11.84) | 0.13 (0.088–0.19) | 2.62 (2.19–3.15) |
| R430Q EMV | 0.017 (0.016–0.022) | 0.14 (0.10–0.20) | 1.05 (0.85–1.30) | 0.24 (0.18–0.33) | 8.56 (5.72–13.90) | 0.11 (0.093–0.12) | 0.056 (0.050–0.064) |
| A/New York/PV01575/2018 N270K/N369K | >30* | 0.041 (0.029–0.056) | >30* | >30* | 19.79 (14.02–30.00) | >30* | 0.064 (0.056–0.073) |
| Wild-type rNA | 0.020 (0.019–0.021) | 0.12 (0.11–0.13) | 0.013 (0.013–0.014) | 0.19 (0.17–0.21) | >10 | 0.027 (0.025–0.028) | ND |
| N88D rNA | 0.0092 (0.0075–0.011) | 0.11 (0.092–0.12) | 0.0057 (0.0034–0.0082) | 0.21 (0.16–0.26) | >10 | 0.027 (0.024–0.030) | ND |
| N270D rNA | 0.0081 (0.0069–0.0093) | 0.42 (0.29–0.61) | 9.20 (5.11–34.06)*** | >10*** | >10 | 3.66 (2.92–4.64) | ND |
| Q313K rNA | 0.019 (0.017–0.021) | 5.89 (4.74–7.46)** | 0.0126 (0.0095–0.017) | 0.17 (0.14–0.21) | >10 | 0.024 (0.022–0.025) | ND |
| Q313R rNA | 0.015 (0.013–0.017) | 0.56 (0.46–0.68) | 0.013 (0.011–0.014) | 0.17 (0.16–0.20) | >10 | 0.024 (0.023–0.025) | ND |
| S364N rNA | 0.38 (0.23–0.61) | 0.088(0.074–0.10) | 0.0192 (0.013–0.028) | 0.12 (0.064–0.22) | >10 | 0.015 (0.012–0.020) | ND |
| N369T rNA | 0.044 (0.041–0.048) | 0.19 (0.17–0.22) | 0.0198 (0.017–0.023) | 0.27 (0.22–0.32) | >10 | 0.033 (0.031–0.035) | ND |
| S364N/N369T rNA | 0.18 (0.11–0.31) | 0.11 (0.075–0.17) | 0.019 (0.015–0.025) | 0.074 (0.045–0.12) | >10 | 0.017 (0.015–0.019) | ND |
| R430Q rNA | 0.011 (0.0012–0.10) | 0.24 (0.13–0.44) | 0.0006384 (2.83 × 10−6–0.14) | 0.16 (0.041–0.69 | >10 | 0.0167 (0.015–0.018) | ND |
| G454D rNA (corresponds to irrelevant IgG control virus A) | 0.0066 (0.0051–0.0084 | 0.038 (0.030–0.049) | 0.01237 (0.008875–0.01670 | 0.061 (0.025–0.13) | >10 | 0.020 (0.016–0.025) | ND |
a
IC50 values (with 95% confidence intervals) are listed in micrograms per milliliter. Thirty micrograms per milliliter was the highest mAb concentration assessed for EMVs, while 10 μg/mL was the highest mAb concentration tested for rNAs. Boldface type indicates significant differences in IC50s between irrelevant IgG control virus B and the EMV (*, P < 0.05; **, P < 0.01; ***, P < 0.001). ND, not determined.