FIG 4.

Phosphomimetic mutations of S210 inhibit nuclear localization and MT association of Nishigahara P3 protein. (A) COS-7 cells were transfected to express the indicated proteins before CLSM analysis of living cells 24 h later. (B) Images such as those shown in panel A were analyzed to determine the ratio of nuclear to cytoplasmic fluorescence, corrected for background fluorescence (mean Fn/c ± SEM, n ≥ 88 cells for each condition, from two separate assays). ***, P < 0.001; ns, not significant.