FIG 5.

Quantitative dSTORM analysis indicates that phosphomimetic mutations of S210 inhibit MT bundling by Nishigahara P3 protein. (A) dSTORM images of immunostained β-tubulin in COS-7 cells expressing the indicated proteins, with example MTfds indicated by white arrowheads. (B) Frequency distribution of MTfds calculated for each protein. A histogram shows the percentages of MTs in the indicated range of MTfds, calculated using measurements of n = 209 [GFP-Ni-P3], 235 [GFP-Ni-P3-S210A], 275 [GFP-Ni-P3-S210D], 276 [GFP-Ni-P3-S210E], and 245 [GFP alone] MTs in images from five cells for each protein. (C) Tukey box plots calculated using the MTfd measurements described for panel B. ***, P < 0.001; ns, not significant.