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Binding kinetics of wild type and phosphomimetic S210E PCTD for N-pep
| PCTD | KD (μM)a | KD (μM)b | Koff (s−1)b | Kon (s−1 μM−1)b |
|---|---|---|---|---|
| Wild type | 88 ± 4 | 79 ± 3 | 1,970 ± 102 | 25.0 |
| S210E | 140 ± 6 | 123 ± 3 | 2,237 ± 98 | 18.2 |
KD determined by saturation binding curve analysis using Xcrvfit.
KD, on-rate and off-rate were determined from lineshape analysis using TITAN.
