FIG 2.

Sequence properties of SsYkV1. (A) Genomic organization of SsYkV1. The conserved regions of RdRp and the 2A-like motif in the putative polyprotein are marked. The potential proteins cleaved by the 2A-like enzyme are shown as purple and light green rectangles. Each lower gray rectangle represents a peptide identified by LC-MS/MS. (B) Genomic coverage of SsYkV1 was obtained by mapping the ribodepleted RNA reads to the viral genome. (C) Multiple alignments of the amino acid sequences of RdRps encoded by SsYkV1 and other selected viruses. The seven conserved regions (motifs A to G) are indicated. (D) Matrix based on the pairwise comparison of identities obtained using BLASTp of the NCBI. The color scale indicates the corresponding identities. (E) Multiple-sequence alignments of 2A-like motifs in the members of the family Yadokariviridae and other related viruses. The red arrow represents the hypothetical cleavage site. Sequence information for all selected viruses (C, D and E) is supplied in Table S1 in the supplemental material.