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Effect of RNase R treatment on circular RNA enrichment. Agarose gel electrophoresis of RT-PCR products of circular (A) and linear (B) gga-WDR62 and MDV-meq was obtained from three samples untreated or treated with RNase R before the reverse transcription procedure as indicated. Ladder: SmartLadder SF 100 bp to 1kb (Eurogentec). Asterisks (*) indicate rolling circle amplicons.
