TABLE 1.
Primers used in this studya
| No. PCR | PCR target | Orientation | Sequence (5′–3′) | Position on TRL or IRS | Position on IRL or TRS |
|---|---|---|---|---|---|
| 1 | pp14-ex1 | Fwd | CGATCCCCGATATATTTCCT | 13791-13772 | 129209-129228 |
| Rev | CGTGCCATTCTGAGAGAGCA | 13794-13813 | 129206-129187 | ||
| 2 | pp14-ex1 | Fwd | GAGCTGAATTTCTCCCTTCATC | 13743-13722 | 129257-129278 |
| Rev | CGTGCCATTCTGAGAGAGCA | 13794-13813 | 129206-129187 | ||
| 3 | pp14-ex2 | Fwd | GTCGATTGACACGGCTCTG | 10867-10849 | 131473-131491 |
| Rev | GACGTTGATGGAGGAGTTGC | 10927-10946 | 131413-131394 | ||
| 4 | ERL-ex9 | Fwd | GCTTCCGTCTTTGTACATGATAA | 10921-10899 | 131419-131441 |
| Rev | GACGTTGATGGAGGAGTTGC | 10927-10946 | 131413-131394 | ||
| 5 | pp14-ex3 | Fwd | CAACTAGGAACGGAACATCAAGT | 9748-9726 | 132592-132614 |
| Rev | AGTGGGTCCGCAGTCAATGC | 9774-9793 | 132566-132547 | ||
| 6 | pp14-ex3 | Fwd | GGTTCTGGCAGAGATTCCAC | 9793-9774 | 132704-132723 |
| Rev | AGTGGGTCCGCAGTCAATGC | 9774-9793 | 132566-132547 | ||
| 7 | ERL-ex8 | Fwd | CCATCTCGAAACGGATACTGC | 9771-9751 | 132569-132589 |
| Rev | GGTCTGCAACGATATCACAGC | 9800-9820 | 132540-132520 | ||
| 8 | ERL-ex7 | Fwd | AGATGTTGTAGGGTTCGAGAGG | 9180-9159 | 133159-133180 |
| Rev | ACGACCGACAGAGAAAGTTGG | 9249-9269 | 133090-133070 | ||
| 9 | RLORF6/ERL-ex6 | Fwd | AACGATTGCGGAAGTACGGC | 8686-8667 | 133653-133672 |
| Rev | CTGCAAACTATATCGGAATGCG | 8853-8874 | 133486-133465 | ||
| 10 | ERL-ex5 | Fwd | TCGTGACATTTAGAACCAAAGTG | 7542-7520 | 134797-134819 |
| Rev | AGCCTTAAGTAGAATAACTGGCG | 7599-7621 | 134740-134718 | ||
| 11 | miRNA cluster M9M4 | Fwd | CTGGGGGAAATGATCGCTGG | 6266-6247 | 136074-136093 |
| Rev | ATACGCGGTGTCACGAAGACCGA | 6518-6540 | 135822-135800 | ||
| 12 | Meq | Fwd | CACCCCTTCCCTGACGGCCTATC | 5690-5668 | 136652-136674 |
| Rev | GTGCCCGCCTTCTCCCTGGTATAC | 5843-5866 | 136499-136476 | ||
| 13 | ERL-ex3 | Fwd | TATGCCCTACAGTCCCGCTG | 5799-5780 | 136543-136562 |
| Rev | GAACGGTCACAATTCACCTGTC | 6005-6026 | 136337-136316 | ||
| 14 | ERL-ex2 | Fwd | TCAGCAGCACATCGTCTATGC | 4787-4767 | 137555-137575 |
| Rev | CCGAATACAAGGAATCCTGTTC | 4880-4901 | 137462-137441 | ||
| 15 | RLORF5a/ERL-ex1 | Fwd | TATGCTTTCTCTACGATGTGGCA | 3673-3651 | 138668-138690 |
| Rev | CGCAGATTACTCCTGCATAAGC | 3857-3878 | 138484-138463 | ||
| 16 | RLOF5a | Fwd | AGAGCATGAAAATTAAATCGTAGC | 3419-3396 | 138922-138945 |
| Rev | CCCTTCCCGTTCACTCTTTC | 3487-3506 | 138854-138835 | ||
| 17 | RLORF4 | Fwd | AGACCCAATAACAGGGAAATCG | 2711-2690 | 139629-139650 |
| Rev | GGGCATAAACTATAGCATCGAA | 2798-2819 | 139542-139521 | ||
| 18 | vIL-8-ex2 | Fwd | CTGTTGACGTGATACCACCG | 2285-2266 | 140055-140074 |
| Rev | ACAGCGAGACTCTCCAGTGATA | 2348-2369 | 139992-139971 | ||
| 19 | vIL-8-ex3 | Fwd | ACACAATTGAGCCCACACCTC | 1983-1963 | 140357-140377 |
| Rev | CACATACCTTCCTGTTCTTCTTGAG | 2107-2131 | 140233-140209 | ||
| 20 | LAT ex1 | Fwd | CCGCAGGTCTCTCGGACTAG | 142813-142832 | 177550-177531 |
| Rev | CGCCAAACTTGTGCCAAAC | 142773-142755 | 177590-177608 | ||
| 21 | LAT ex2 | Fwd | TTCTTTTGCGTCTGCCGAC | 144509-144527 | 175854-175836 |
| Rev | TACGATCTATCGGACGAACCAC | 144382-144361 | 175981-176002 | ||
| 22 | LAT ex3 | Fwd | CGGGAATGTGAACAACAACC | 145206-145225 | 175157-175138 |
| Rev | GAAGATATGGTAGCCTCAATTCG | 145182-145160 | 175181-175203 | ||
| 23 | LAT ex5 | Fwd | TCGGGTGCTGCTTTCCATC | 145877-145895 | 174486-174468 |
| Rev | TATCTCCACTGACTCGGATG | 145874-145855 | 174489-174508 | ||
| 24 | LAT ex7 | Fwd | GTTTGACCGATCACCGTTCC | 146342-146361 | 174021-174002 |
| Rev | CCGAGGCTATGGCAGACATC | 146234-146215 | 174129-174148 | ||
| 25 | LAT ex9 | Fwd | ATGAAGAGGATGGCGATCTGG | 148764-148784 | 171599-171579 |
| Rev | CGTAAACTGGAAGATGAGGAC | 148566-148546 | 171797-171817 | ||
| 26 | LAT ex11 | Fwd | TCTCAAAAGTAACTTCGCAGCC | 149418-149439 | 171945-171924 |
| Rev | CCACAACCATCTACCACGCTG | 149415-149395 | 170948-170968 | ||
| 27 | LAT ex13 | Fwd | CCACAATTGCAAAGTGCGGTA | 152320-152340 | 168043-168023 |
| Rev | TCGGGCAGCAATCAGATACG | 152277-152258 | 168086-168105 | ||
| 28 | LAT ex15 | Fwd | TCGGGGTTCTGTACGATTGG | 153647-153666 | 166716-166697 |
| Rev | TTTTGTTTACTTGCAGATATGCACC | 153645-153621 | 166718-166742 | ||
| gga-circWDR62 | Fwd | CTCAAGCAGCATTTTGGGACC | NA | NA | |
| Rev | GTCTGGGCTGGAAAGACGAC | NA | NA | ||
| gga-linear WDR62 | Fwd | CTCCTACATCTGTAACTGCCTTGG | NA | NA | |
| Rev | GTATCTTGTGGCTTCGTCAGTACC | NA | NA |
a
Primers were designed according to the sequence of the MDV RB-1B genome.