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. 2022 Apr 11;96(9):e00349-22. doi: 10.1128/jvi.00349-22

FIG 5.

FIG 5

ICP0-dependent and independent suppression of HSV-2 replication and gene expression by miR-138 in Neuro-2a cells. (A) Neuro-2a cells were transfected with 20 nM of the indicated mimic for 24 h and then infected with the indicated virus for 48 h (MOI = 0.2) before viral titer measurements. n = 6 (B) Same as panel A, but HSV2ICP0− virus was used for infection (MOI = 5). n = 4. (C) Neuro-2a cells were transfected with 160 nM of the indicated antagomir for 24 h and then infected with HSV-2 strain 186 for 24 h (MOI = 0.4) before viral titer measurements. n = 3. (D) Same as panel C, but the indicated viruses were used for infection (MOI = 1). n = 5. (E) Neuro-2a cells were infected with the HSV-2 strain 186 (MOI = 0.2) for the indicated times before qRT-PCR analysis of ICP0, TK, and UL19-20 mRNA levels. n = 4. Data are presented as mean values ± SD. Data were analyzed by one-way ANOVA with Dunnett’s multiple-comparison tests (A and B), two-tailed unpaired t-tests (C), or two-way ANOVA with Sidak’s multiple-comparison tests (D and E).