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. 2022 May 6;136(9):657–673. doi: 10.1042/CS20220043

Figure 5. ANGPTL1 suppressed cell migration and invasion through FOXO3a induction in HCT116 cells.

Figure 5

(A) The protein expression of FOXO family was assessed by Western blotting. The expression of α‐tubulin used as an internal loading control. (B) The mRNA expression of FOXO3 was analyzed using RT-PCR analysis. GAPDH was used as reference gene; *, P<0.05 (C) The p-FOXO3a and FOXO3a expression in ANGPTL1-overexpressed cells was performed. The expression of α‐tubulin is as an internal loading control. (D) The expression of FOXO3a in the nucleus and cytosol was assessed by Western blotting. The expression of Lamin A/C used as nuclear internal loading control and α‐tubulin as a cytosol internal loading control. (E) The knockdown efficiencies of FOXO3a were analyzed. The expression of α‐tubulin is as an internal loading control. (F–H) Both migration and invasion abilities were examined and quantified. Results are shown as the mean ± S.D. of three independent experiments. *, P<0.05, compared with indicated cells; scale bar = 100 μm.