FIG. 5.

Time courses of the degradation and assimilation of cellooligosaccharides by each transformant. (A) Aliquots (1 μl) of the culture supernatants after cultivation of strain MT8-1/pBG211 or MT8-1/pCMC11/pBG211 for 0, 6, 12, 18, 24, and 96 h and authentic sugars (S, composed of G₁, G₂, G₃, G₄, G₅, and G₆) were chromatographed as described in the text. The sugars detected were as follows: G₁, glucose; G₂, cellobiose; G₃, cellotriose; G₄, cellotetraose; G₅, cellopentaose; G₆, cellohexaose. (B) The cells of the strains MT8-1/pCMC11 and MT8-1 were suspended in 0.5% cellooligosaccharide solution and incubated at 37°C for the indicated time, and then aliquots (1 μl) of the supernatants were chromatographed. Since the strain MT8-1/pCMC11 could not grow in the cellooligosaccharide-containing medium, CMCase activity against the cellooligosaccharides was examined with a concentrated suspension of the cells.