TABLE 1.

Yields of different scFv constructs expressed in P. mirabilis L VI and in E. coli JM109

scFv antibody	Expression plasmid	Avg concn of scFv in L VI supernatant ± SDa (mg/liter)	Increase in yield of L VI compared to that of JM109b	Increase in specific yield of L VI compared to that of JM109c	Avg amt of purified, highly active scFv ± SDd (mg)
F19	pEA50	47 ± 20	101	18	ND
OS4	pEA40	83 ± 23	160	32	11.92 ± 0.82
H398	pEA11	127 ± 32	67	15	9.20 ± 1.13
TTX	pEA30	200 ± 20	33	17	ND

^aThe contents of soluble scFvs in the supernatants of P. mirabilis L VI cultures were determined by Western blotting in four different experiments. 

^bValues are ratios of ELISA signals per sample volumes, normalized for the volumes of P. mirabilis L VI and E. coli JM109 cultures. 

^cValues are ratios of ELISA signals per sample volumes, normalized for the protein contents of P. mirabilis L VI and E. coli JM109 lysates. 

^dFinal yield of purified scFv from P. mirabilis L VI culture, determined by ELISA and Western blotting of two different purifications. Values are calculated for a 1-liter culture. ND, not determined.