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. 1998 Dec;64(12):4870–4876. doi: 10.1128/aem.64.12.4870-4876.1998

FIG. 1.

FIG. 1

Analysis of PCR-amplified 16S rRNA genes by SSCP on 0.6× MDE polyacrylamide gels, comparing band positions obtained from nondenatured PCR products (lanes 2, 5, and 8), denatured PCR products (lanes 3, 6, and 9), and PCR products after removal of one single strand (lanes 4, 7, and 10). Template DNA for PCRs was obtained from pure cultures of B. subtilis (lanes 2 to 4), S. meliloti (lanes 5 to 7), and P. fluorescens (lanes 8 to 10). Size standard VI (double-stranded DNA; Boehringer) is shown in lane 1.