KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit α-HA-tag, 1:1000 | Cell signaling | 3724S |
| AlexaFluor488-fluorescently-conjugated goat α-rabbit | Life Technologies | A-11070 |
| Bacterial and virus strains | ||
| AAV-hSyn-HI-eGFP-Cre | Addgene | Addgene #: 105540-AAV9 |
| AAV-hSyn-EGFP | Addgene | Addgene #: 50465-AAV9 |
| AAV-hSyn-DIO-mCherry | Addgene | Addgene #: 50459-AAV9 and AAVrg |
| AAV-hSyn-DIO-EGFP | Addgene | Addgene #: 50457-AAVrg |
| AAV-EF1a-Flpo | Addgene | Addgene #: 55637-AAVrg |
| AAV-EF1a-fDIO-Cre | Addgene | Addgene #: 121675-AAV9 |
| AAV-FLEX-tdTomato | Addgene | Addgene #: 28306-PHP.eb |
| AAV-TBG-PI-Cre | Addgene | Addgene #: 107787-AAV8 |
| AAV-TBG-PI-Null | Addgene | Addgene #: 105536-AAV8 |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant human FGF21 | NovoNordisk | |
| PF-05231023 | Pfizer | |
| N-Methyl-D-glucamine | Sigma | Cat#:M2004 |
| KCl | Research Products International | Cat#:P41025–500.0 |
| NaCl | Research Products International | Cat#:S23020–500.0 |
| NaH2PO4 | Research Products International | Cat#:S23185–500.0 |
| NaHCO3 | Research Products International | Ca#:S25060–500.0 |
| HEPES | Research Products International | Cat#:H75030–50.0 |
| Glucose | Research Products International | Cat#:G32045–500.0 |
| MgSO4-7H2O | Research Products International | Cat#:M65240–100.0 |
| CaCl2-2H2O | Research Products International | Cat#:C36200–500.0 |
| Sodium Ascorbate | Research Products International | Cat#:S42175–100.0 |
| Thiourea | Sigma | Cat#:T8656 |
| Sodium Pyruvate | Sigma | Cat#:P2256 |
| Pronase | Roche | Cat#: 10165921001 |
| Fetal Bovine Serum | Gibco | Cat#:26140–079 |
| CNQX | Tocris | Cat#:0190 |
| TTX | Tocris | Cat#:1078 |
| D-AP5 | Tocris | Cat#:0106 |
| Pertussis Toxin | Tocris | Cat#:3097 |
| Actinomycin D | Sigma | Cat#: A1410 |
| Critical commercial assays | ||
| Mouse/Rat FGF21 ELISA | Biovendor | Cat#: RD291108200R |
| BD Rhapsody Whole Transcriptome Analysis Kit | BD Biosciences | Cat#: 633802 |
| Deposited data | ||
| Raw and processed scRNAseq data | Gene Expression Omnibus | GEO: TBD |
| Experimental models: Organisms/strains | ||
| FGF21fl/fl | Markan et al., 2014 | |
| FGF21 LivKO | Markan et al., 2014 | |
| KLBfl/fl | Ding et al., 2012 | |
| Ai14-tdTomato | The Jackson Laboratory | 007914 |
| KLB-Cre | Jensen-Cody and Flippo et al., 2020 | |
| KLB loxTB mice | This paper | |
| Oligonucleotides | ||
| Klb: 5' - GATGAAGAATTTCCTAAACCAGGTT-3' , 5' - AACCAAACACGCGGATTTC −3' | von Holstein-Rathlou et al. 2016 | |
| Fgf21: 5'-CCTCTAGGTTTCTTTGCCAACAG-3', 5'-AAGCTGCAGGCCTCAGGAT-3'; | von Holstein-Rathlou et al. 2016 | |
| Cre: 5'-TGTTGCCGCGCCATCTG-3', 5'-TTGCTTCAAAAATCCCTTCCA-3' | von Holstein-Rathlou et al. 2016 | |
| U36B4: 5'-CGTCCTCGTTGGAGTGACA-3', 5'-CGGTGCGTCAGGGATTG-3' | von Holstein-Rathlou et al. 2016 | |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Seurat | Stuart and Butler et al., 2019 | https://satijalab.org/seurat/ |
| BD Rhapsody Analysis pipeline | BD | sbgenomics.com/bdgenomics |
| Axon pCLAMP 11.3 | Molecular Devices | N/A |
| R | The R Project for Statistical Computing |
https://www.r-project.org/ |
| Graphpad Prism 7 | Graphpad | http://www.graphpad.com/scientific-software/prism/ |