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. 2022 Apr 27;15:874903. doi: 10.3389/fnmol.2022.874903

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Copyright © 2022 Zhou, Xu, Chao, Chen, Li, Shi, Pei, Dai, Ji, Ji and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of sEVs isolated from human serum. (A) The TEM image shows the spherical morphology of sEVs with a diameter of approximately 100 nm, bar = 100 nm. (B) NTA analysis plot illustrates the size distribution and concentration of the sEVs isolated human serum. (C) Proteins from serum, sEVs-depleted serum, and sEVs were separated by SDS-PAGE and stained by Coomassie blue. 40 μg proteins from serum and sEVs depleted serum were loaded. The amount of sEVs protein was adjusted by the serum volume corresponding to 40 μg serum protein. (D) The expression of CD9, CD81, and CD63, markers of exosomes, was detected by western blotting. Lane 1: total serum; lane 2: sEVs-depleted serum; lane 3: serum sEVs.