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. 2022 Apr 27;15:874903. doi: 10.3389/fnmol.2022.874903

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Copyright © 2022 Zhou, Xu, Chao, Chen, Li, Shi, Pei, Dai, Ji, Ji and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the upregulation of the four brain-specific miRNAs in stroke patients. The expression of hsa-miR-9-3p (A), hsa-miR-124-3p (B), hsa-miR-143-3p (C), and hsa-miR-93-5p (D) was assessed using qRT-PCR in the individual samples for small RNA sequencing. IS patients, n = 40, healthy controls (HCs), n = 33. The Ct value of target miRNAs was normalized against Cel-mir-39 and compared to the mean of healthy controls. Data are presented as Mean ± SEM. Unpaired Student’s test vs. healthy control (**P < 0.01, ***P < 0.001, ****P < 0.0001).