TABLE 1.
Strains and plasmids used in this studya
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| Mv1190λpir | F′ (traD36 proAB+ lacZΔM15 lacIq) thi Δ(lac-proAB) Tn10λpir | 9 |
| JM109 | F′ (traD36 proAB+ lacZΔM15 lacIq) thi Δ(lac-proAB) recA1 gyrA96 | 19 |
| Pseudomonas putida | ||
| CMC4 | (pWW0) Kmr 3MB+ mini-Tn5-Km (xylS Pm::lacI PA1-04/03::gef) | 23 |
| CMC12 | (pWW0) Kmr Telr 3MB+ EEZ29::mini-Tn5-Tel (PA1-04/03::gene E) | This study |
| CMC13 | (pWW0) Telr Kmr 3MB+ EEZ29::mini-Tn5-Tel | This study |
| EEZ15K-3 | (pWW0) Kmr 3MB+ mini-Tn5-Km | 29 |
| EEZ29 | (pWW0) pCC102 Kmr 3MB+ | 31 |
| Plasmids | ||
| pCC102 | KmrxylS2 Pm::lacI | 1 |
| pJMSB4 | Apr Telr mini-Tn5-Tel R6K oriV mob+ | 33 |
| pMCC26 | pUHE24-1 derivative; Apr Cms | This study |
| pMCC27 | Apr PA1-04/03::gene E | This study |
| pMCC30 | Apr PA1-04/03::gene E fragment of pMCC27 inserted between SalI and HindIII sites of pUC18Not | This study |
| pMCC31 | Apr Telr mini-Tn5 PA1-04/03::gene E R6K oriV mob+ | This study |
| pRK600 | Cmr ColE1 oriV RK2 mob+ tra+ | 12 |
| pUC18Not | Apr | 2, 9 |
| pUHE24-1 | Apr Cmr ColE1 oriV | 16 |
| pWW0 | IncP9 mob+ tra+ 3MB+ | 39 |
a
Kmr, Apr, Cmr, and Telr indicate resistance to kanamycin, ampicillin, chloramphenicol, and potassium tellurite, respectively; Cms indicates sensitivity to chloramphenicol; 3MB+ indicates the ability to grow on 3-methylbenzoate. PA1-04/03 is a synthetic lactose promoter (16).