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[Preprint]. 2022 May 2:rs.3.rs-1576788. [Version 1] doi: 10.21203/rs.3.rs-1576788/v1

Figure 1. CNBP inhibits SARS-CoV-2 replication in vitro.

Figure 1.

(A) hACE2-A549 cells were transfected with a Flag-CNBP expression plasmid or control, infected with SARS-CoV-2 for 24 hrs, and dsRNA was visualized by immunofluorescence with anti-J2 antibody (green). (B-D) Normalized SARS-CoV-2 RNA levels of NP (B) and NSP14 (C) as well as the SARS-CoV-2 titers (D) in hACE2-A549 cells transfected with Flag-CNBP plasmid and infected with SARS-CoV-2. (E-I) CNBP pKO and Cas9 Ctl A549 cells were infected with SARS-CoV-2 at an MOI of 0.01. At 24 h post-infection, western blotting with viral NP protein expression (E), immunofluorescence staining with anti-J2 antibody (F), qPCR analysis of vRNA levels of NP (G) and NSP14 (H) as well as the viral titers assessed by plaque assay (I) in the supernatants were determined.