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[Preprint]. 2022 May 2:rs.3.rs-1576788. [Version 1] doi: 10.21203/rs.3.rs-1576788/v1

Extended Data Figure 3: SARS-CoV-2 N undergoes LLPS.

Extended Data Figure 3:

(A and B) RIP assay with hACE2-A549 cell lysates prepared after 24 h of infection with OC-43 virus or respiratory syncytial virus (RSV) by using anti-CNBP or control immunoglobulin. Immunoprecipitated OC-43 virus RNA (A) or RSV RNA (B) was quantified by RT-qPCR. (C) Nucleoprotein LLPS in the presence of SARS-CoV2 genome RNA observed under bright field using a confocal microscope and were disrupted in the presence of 1,6-hexanediol. (D) 20 μM CNBP fails to undergo LLPS. (E) The turbidity of CNBP was measured by absorbance at 350 nm.