Table 2.

Spearman's (non-parametric) rank-order correlations between α-diversity metrics in gut prokaryotic microbiota in Japanese beetle (P. japonica Newman) larvae and host soil physicochemical characteristics across locations.

	Midgut								Hindgut
	Observed ASVsa		Evenness		Shannon diversity		Faith's PDb		Observed ASVsa		Evenness		Shannon diversity		Faith's PDb
Soil variable	rs	p	rs	p	rs	p	rs	p	rs	p	rs	p	rs	p	rs	p
CECc	−0.236	0.303	−0.299	0.188	−0.350	0.120	−0.260	0.256	−0.083	0.722	−0.448	0.042	−0.342	0.129	−0.075	0.748
OMd	0.042	0.858	0.157	0.498	0.093	0.688	0.004	0.986	−0.298	0.190	−0.212	0.356	−0.321	0.155	−0.413	0.063
pH	−0.236	0.303	−0.271	0.234	−0.338	0.134	−0.232	0.312	−0.232	0.312	−0.436	0.048	−0.405	0.069	−0.201	0.383
Sand	0.315	0.164	0.559	0.008	0.502	0.020	0.226	0.324	−0.020	0.932	−0.052	0.824	−0.060	0.798	−0.054	0.818
WHCe	−0.362	0.107	−0.362	0.107	−0.389	0.081	−0.334	0.139	−0.389	0.081	−0.338	0.134	−0.444	0.044	−0.358	0.111

^a ASVs, Amplicon sequence variants;

^b Faith's PD, Faith's phylogenetic distance;

^c CEC, Cation Exchange Capacity (meq/100 g);

^d OM, Organic matter percentage;

^eWHC, Water Holding Capacity at 1/3 Bar. Boldface values indicate significant correlation (p ≤ 0.05).