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. 2022 Apr 19;9:826505. doi: 10.3389/fmolb.2022.826505

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Copyright © 2022 Fuks, Falkner, Schwierz and Hengesbach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative structures of the three states from coarse-grained simulations at different Mg²⁺ concentrations. The P1 and P2 stem-loops are coloured in purple and blue respectively, the anti-SD sequence is coloured in yellow and the rest of the linker is coloured in grey. The corresponding secondary structure is shown below each three-dimensional structure. Positions of Cy3 and Cy5 fluorophores of the smFRET construct are depicted as a green and red star, respectively. The calculated FRET efficiency (E_FRET) of each structure averaged over different dye orientations is given. R_G describes the radius of gyration.