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(A) Example of a FRET trace of a static molecule in the M-state. (B) Three state kinetic model. (C) Examples of dwell time plots used to determine transition rates. smFRET trajectories for each condition were fitted for three states with HaMMy and resulting dwell times were fitted with a monoexponential decay. (D) Change of transition rates with increasing Mg2+ concentration of the transitions of the U to M (dark grey) and K to M (light grey).
