TABLE 2.
Transition rates derived from dwell time fits at various Gdm+ concentrations at 1 mM Mg2+.
| Transition | 0 | 30 nM | 1 µM | 30 µM | 1 mM | 30 mM |
|---|---|---|---|---|---|---|
| kU,K [s−1] | 3.2 ± 0.04 (n = 737) | 3.5 ± 0.04 (n = 730) | 2.9 ± 0.04 (n = 929) | 3.7 ± 0.07 (n = 934) | 3.7 ± 0.04 (n = 682) | 6.4 ± 0.09 (n = 933) |
| kK,U [s−1] | 1.6 ± 0.03 (n = 729) | 1.0 ± 0.05 (n = 696) | 0.8 ± 0.05 (n = 903) | 1.9 ± 0.03 (n = 846) | 1.2 ± 0.06 (n = 632) | 1.4 ± 0.03 (n = 930) |
| kU,M [s−1] | 1.6 ± 0.04 (n = 79) | 2.4 ± 0.11 (n = 95) | 1.5 ± 0.05 (n = 917) | 1.4 ± 0.05 (n = 215) | 3.3 ± 0.09 (n = 107) | 5.0 ± 0.19 (n = 49) |
| kM,U [s−1] | 0.6 ± 0.03 (n = 86) | 0.3 ± 0.04 (n = 129) | 0.3 ± 0.03 (n = 223) | 0.4 ± 0.03 (n = 303) | 0.4 ± 0.04 (n = 157) | 0.6 ± 0.11 (n = 51) |
| kK,M [s−1] | 0.9 ± 0.05 (n = 71) | 0.8 ± 0.05 (n = 143) | 0.7 ± 0.06 (n = 162) | 1.0 ± 0.07 (n = 428) | 1.5 ± 0.08 (n = 402) | n.d. (n = 26) |
| kM,K [s−1] | 0.7 ± 0.04 (n = 64) | 0.2 ± 0.03 (n = 109) | 0.2 ± 0.02 (n = 135) | 0.3 ± 0.02 (n = 340) | 0.4 ± 0.02 (n = 353) | n.d. (n = 23) |
In cases where a reliable fit could not be obtained, i.e. due to a low number of transitions (n), the rates were not determined (n.d.).