Figure 3.

AMH upregulated SMAD signal pathway and MALAT1 expression. (A) KGN cells were treated with a range of AMH dose (5 to 50 ng/ml) for 24 h. The expression level of MALAT1 was determined by RT-qPCR (^* p < 0.05; ^*** p < 0.01). (B) Graph showing the expression level of MALAT was gradually increased in KGN cells treated with 20 ng/ml AMH and was determined by RT-qPCR (^* p < 0.05; ^*** p < 0.01). (C) Representative Western blot images using anti-pSMAD 1/5, anti-SMAD1, and anti-SMAD5 antibodies on total protein lysates extracted from KGN cells treated with AMH (20 ng/ml) for 120 minutes. Quantitative analysis of protein bands was performed by ImageJ. ^* p < 0.05; ^** p < 0.01. (D) Scheme graph of MALAT-induced proliferation decreased in granulosa cells. AMH upregulated the expression of MALAT via activing phosphorylated SMAD1/5, leading to suppress granulosa cell.