Figure 4.

Microscopic examination of images of representative tissue specimens stained with Masson’s Trichrome captured at 40x magnification (A, D). Large and small rectangular areas were captured at 100x (B, E) and 400x (C, F), respectively. Animals treated with LXA₄-ME after cleft lip repair showed decreased density of collagen fibers compared to control treated with PBS. A higher number of hair follicles was observed in histological sections of animals treated with LXA₄-ME (D, E) compared to control (A, B), indicating wound healing closer to normal skin than scar tissue. Muscle fibers and cells were more frequently identified within collagen fibers in animals treated with LXA₄-ME (D, F), while scar tissue and dense collagen interfered with muscle regeneration in control animals (A,C). The scale bars represent 500 um (A, D), 200 um (B, E) and 50 um (C, F).