Figure 5.

Automated quantification of collagen fiber density of JPEG images of representative tissue specimens stained with Masson’s Trichrome captured at 200x magnification (A, D). Collagen fibers are coded red in images illustrating collagen density (B, E) or orange in whole field images (C, F). Automated quantification of collagen fiber density using custom-written code in Matlab showed reduced collagen fiber density in animals treated with LXA4-ME (median=0.37, 25^th; 75^th percentile=0.34, 0.42) (E, F) compared to control animals treated with PBS (median=0.67, 25^th; 75^th percentile=0.59, 0.74) (B, C). The average of three regions per animal were used for statistical analysis (G) (*P < 0.05, Mann-Whitney U test; n=4 animals/group). The color bars for panel b and e are ranging from 0-1. The scale bars represent 100 um.