Table 2.
Correlation of early gene expression with vaccine-induced Env-specific IgG.
| Parameter | Spearman Correlation | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| week 14 | week 20 | week 32 | week 34 | |||||||||
| r | p | q | r | p | q | r | p | q | r | p | q | |
| D1 DEG | ||||||||||||
| SERPING1 | 0.3464 | 0.2061 | 0.6314 | 0.0250 | 0.9336 | 0.9682 | 0.4536 | 0.0915 | 0.5484 | 0.3393 | 0.2161 | 0.6415 |
| C3AR1 | 0.5321 | 0.0438 | 0.5158 | 0.3607 | 0.1870 | 0.6212 | 0.5964 | 0.0213 | 0.5158 | 0.3821 | 0.1607 | 0.6147 |
| IL1RN | 0.4036 | 0.1370 | 0.5932 | 0.1500 | 0.5934 | 0.8379 | 0.4286 | 0.1127 | 0.5771 | 0.3714 | 0.1735 | 0.6182 |
| TNFAIP6 | 0.4679 | 0.0808 | 0.5470 | 0.1893 | 0.4983 | 0.7907 | 0.3607 | 0.1870 | 0.6212 | 0.4643 | 0.0834 | 0.5470 |
| PLAUR | -0.0750 | 0.7925 | 0.9346 | -0.1857 | 0.5067 | 0.7907 | -0.1357 | 0.6297 | 0.8695 | -0.0107 | 0.9744 | 0.9907 |
| FPR2 | 0.5071 | 0.0562 | 0.5158 | 0.2107 | 0.4498 | 0.7820 | 0.4071 | 0.1334 | 0.5900 | 0.4464 | 0.0972 | 0.5625 |
| SOCS3 | 0.4893 | 0.0666 | 0.5318 | 0.2571 | 0.3538 | 0.7378 | 0.5000 | 0.0602 | 0.5158 | 0.3107 | 0.2592 | 0.6848 |
| OAS1 | 0.3571 | 0.1917 | 0.6278 | 0.0821 | 0.7728 | 0.9263 | 0.3071 | 0.2649 | 0.6973 | 0.3607 | 0.1870 | 0.6213 |
| TNFSF10 | 0.4929 | 0.0644 | 0.5207 | 0.3000 | 0.2767 | 0.6999 | 0.6786 | 0.0068 | 0.4927 | 0.4071 | 0.1334 | 0.5900 |
| CCR1 | 0.4000 | 0.1408 | 0.5999 | 0.0143 | 0.9642 | 0.9833 | 0.2107 | 0.4498 | 0.7820 | 0.4357 | 0.1063 | 0.5737 |
| LILRA3 | 0.6036 | 0.0195 | 0.5158 | 0.3500 | 0.2012 | 0.6314 | 0.5214 | 0.0488 | 0.5158 | 0.5393 | 0.0406 | 0.5158 |
| IFITM1 | 0.5321 | 0.0438 | 0.5158 | 0.2536 | 0.3607 | 0.7379 | 0.6036 | 0.0195 | 0.5158 | 0.4607 | 0.0861 | 0.5470 |
| ARG2 | 0.4821 | 0.0711 | 0.5358 | 0.1821 | 0.5150 | 0.7960 | 0.3929 | 0.1485 | 0.6004 | 0.3964 | 0.1446 | 0.6003 |
| CD82 | 0.4179 | 0.1227 | 0.5900 | 0.2107 | 0.4498 | 0.7820 | 0.3464 | 0.2061 | 0.6313 | 0.2321 | 0.4039 | 0.7633 |
| IRF7 | 0.5607 | 0.0322 | 0.5158 | 0.2500 | 0.3677 | 0.7433 | 0.4429 | 0.1002 | 0.5650 | 0.5107 | 0.0543 | 0.5158 |
| IFI35 | 0.2607 | 0.3469 | 0.7378 | -0.2250 | 0.4189 | 0.7633 | 0.0357 | 0.9031 | 0.9557 | 0.2964 | 0.2827 | 0.6999 |
| NFKBIA | 0.4429 | 0.1002 | 0.5650 | 0.1571 | 0.5756 | 0.8341 | 0.3750 | 0.1692 | 0.6169 | 0.3571 | 0.1917 | 0.6277 |
| CSF3R | 0.5500 | 0.0362 | 0.5158 | 0.2250 | 0.4189 | 0.7633 | 0.4357 | 0.1063 | 0.5737 | 0.5071 | 0.0562 | 0.5158 |
| DDIT3 | 0.5214 | 0.0488 | 0.5158 | 0.2036 | 0.4657 | 0.7881 | 0.3000 | 0.2767 | 0.6999 | 0.5607 | 0.0322 | 0.5158 |
| GP1BB | 0.2036 | 0.4657 | 0.7881 | 0.1143 | 0.6858 | 0.8988 | 0.1500 | 0.5934 | 0.8379 | 0.2607 | 0.3469 | 0.7378 |
| CLU | 0.0786 | 0.7827 | 0.9263 | 0.0357 | 0.9031 | 0.9557 | 0.1857 | 0.5067 | 0.7907 | 0.0464 | 0.8726 | 0.9547 |
| TNFAIP3 | 0.5107 | 0.0543 | 0.5158 | 0.2036 | 0.4657 | 0.7810 | 0.3964 | 0.1446 | 0.6003 | 0.4107 | 0.1297 | 0.5900 |
| IL1RL1 | 0.3643 | 0.1824 | 0.6212 | -0.0750 | 0.7925 | 0.9346 | 0.3107 | 0.2592 | 0.6848 | 0.4786 | 0.0735 | 0.5358 |
| HLA-DMA | -0.2607 | 0.3469 | 0.7378 | 0.1000 | 0.7208 | 0.9098 | -0.2214 | 0.4266 | 0.7633 | -0.2143 | 0.4407 | 0.7785 |
| KIT | -0.1000 | 0.7241 | 0.9098 | -0.3214 | 0.2424 | 0.6804 | -0.1464 | 0.6024 | 0.8473 | 0.1000 | 0.7241 | 0.9098 |
| D1 Shared Pathway Genes c | ||||||||||||
| IL2RA | 0.3429 | 0.2111 | 0.6408 | 0.1179 | 0.6763 | 0.8933 | 0.2357 | 0.3966 | 0.7633 | 0.2179 | 0.4342 | 0.7750 |
| CSF2RB | 0.4821 | 0.0711 | 0.5358 | 0.2214 | 0.4266 | 0.7633 | 0.5036 | 0.0582 | 0.5158 | 0.4071 | 0.1333 | 0.5900 |
| FCGR1A | 0.2071 | 0.4578 | 0.7876 | 0.2821 | 0.3074 | 0.7138 | 0.2893 | 0.2949 | 0.7046 | 0.1857 | 0.5067 | 0.7907 |
| DUSP4 | -0.0464 | 0.8764 | 0.9547 | 0.1429 | 0.6115 | 0.8548 | 0.1821 | 0.5151 | 0.7960 | -0.0786 | 0.7827 | 0.9262 |
| HLA-DQB1 | -0.5714 | 0.0286 | 0.5158 | -0.3464 | 0.2061 | 0.6314 | -0.6036 | 0.0195 | 0.5158 | -0.4107 | 0.1297 | 0.5900 |
| HLA-DRB1 | 0.1000 | 0.7241 | 0.9098 | 0.1071 | 0.7041 | 0.9060 | 0.2393 | 0.3892 | 0.7592 | 0.0393 | 0.8929 | 0.9547 |
| D1 Cytokine Genes d | ||||||||||||
| IFNA2 | -0.1857 | 0.5067 | 0.7906 | -0.2714 | 0.3269 | 0.7335 | -0.2429 | 0.3820 | 0.7548 | -0.0036 | 0.9948 | 0.9999 |
| IL6 | -0.0321 | 0.9132 | 0.9558 | -0.0857 | 0.7630 | 0.9220 | -0.0250 | 0.9336 | 0.9682 | 0.0321 | 0.9132 | 0.9557 |
| IL18 | 0.1964 | 0.4819 | 0.7881 | -0.1036 | 0.7144 | 0.9098 | 0.2321 | 0.4039 | 0.7633 | 0.1786 | 0.5235 | 0.7960 |
| IFNG | -0.3536 | 0.1964 | 0.6314 | -0.3250 | 0.2370 | 0.6739 | -0.1857 | 0.5067 | 0.7907 | -0.2750 | 0.3203 | 0.7310 |
| MCP1 | 0.0786 | 0.7827 | 0.9262 | -0.0786 | 0.7827 | 0.9262 | -0.1179 | 0.6763 | 0.8933 | 0.1179 | 0.6763 | 0.8933 |
| D1 Cytokine Proteins | ||||||||||||
| IFN-α | -0.3750 | 0.1692 | 0.6169 | -0.1214 | 0.6669 | 0.8932 | -0.3464 | 0.2061 | 0.6314 | -0.2571 | 0.3538 | 0.7378 |
| IL-6 | -0.4540 | 0.0905 | 0.5470 | -0.3843 | 0.1573 | 0.6147 | -0.5290 | 0.0447 | 0.5158 | -0.5827 | 0.0248 | 0.5158 |
| IL-18 | -0.2643 | 0.3402 | 0.7378 | -0.2643 | 0.3402 | 0.7378 | -0.3107 | 0.2592 | 0.6848 | -0.2393 | 0.3892 | 0.7592 |
| IFN-γ | -0.2073 | 0.4553 | 0.7873 | -0.2288 | 0.4090 | 0.7633 | -0.0447 | 0.8753 | 0.9547 | -0.0840 | 0.7654 | 0.9220 |
| MCP-1 | -0.2321 | 0.4039 | 0.7633 | -0.3214 | 0.2425 | 0.6804 | 0.0036 | 0.9948 | 0.1000 | -0.4786 | 0.0734 | 0.5358 |
| IL-1RA | 0.1786 | 0.5235 | 0.7960 | -0.1607 | 0.5667 | 0.8284 | -0.0429 | 0.8828 | 0.9547 | 0.1214 | 0.6669 | 0.8932 |
| D3 DEG | ||||||||||||
| CLU | -0.2396 | 0.4086 | 0.7633 | 0.0418 | 0.8915 | 0.9547 | -0.4198 | 0.1368 | 0.5932 | -0.2263 | 0.4356 | 0.7753 |
| GP1BB | -0.2835 | 0.3253 | 0.7335 | 0.1165 | 0.6930 | 0.9029 | -0.4286 | 0.1281 | 0.5900 | -0.2440 | 0.3998 | 0.7633 |
| BCL2L1 | -0.0637 | 0.8319 | 0.9451 | 0.5472 | 0.0458 | 0.5158 | 0.2659 | 0.3573 | 0.7378 | -0.1472 | 0.6158 | 0.8590 |
| IL1RL1 | -0.5297 | 0.0544 | 0.5158 | -0.1956 | 0.5022 | 0.7907 | -0.1165 | 0.6930 | 0.9029 | -0.6483 | 0.0144 | 0.5158 |
| ILR2 | -0.2967 | 0.3026 | 0.7047 | -0.2483 | 0.3911 | 0.7606 | -0.0549 | 0.8557 | 0.9506 | -0.4374 | 0.1198 | 0.5900 |
| D3 Shared Pathway Genes | ||||||||||||
| HLA-C | -0.1253 | 0.6706 | 0.8933 | -0.2615 | 0.3656 | 0.7433 | -0.2747 | 0.3411 | 0.7378 | 0.2396 | 0.4086 | 0.7633 |
| FCGR1A | -0.2527 | 0.3825 | 0.7548 | -0.0637 | 0.8319 | 0.9451 | 0.1033 | 0.7270 | 0.9101 | -0.2703 | 0.3492 | 0.7378 |
| D3 Cytokine Genes | ||||||||||||
| IFNA2 | -0.4330 | 0.1239 | 0.5900 | -0.4154 | 0.1412 | 0.5999 | 0.0242 | 0.9396 | 0.9729 | -0.3670 | 0.1973 | 0.6314 |
| IL6 | -0.2044 | 0.4827 | 0.7881 | -0.0769 | 0.7965 | 0.9360 | 0.0593 | 0.8438 | 0.9484 | -0.2044 | 0.4827 | 0.7881 |
| IL18 | -0.7231 | 0.0047 | 0.4927 | -0.4725 | 0.0905 | 0.5470 | -0.2703 | 0.3492 | 0.7378 | -0.6879 | 0.0082 | 0.4927 |
| IFNG | -0.3978 | 0.1602 | 0.6147 | -0.1516 | 0.6051 | 0.8494 | -0.0461 | 0.8795 | 0.9547 | -0.4725 | 0.0905 | 0.5470 |
| MCP1 | -0.5516 | 0.0438 | 0.5158 | -0.2747 | 0.3411 | 0.7378 | -0.2176 | 0.4541 | 0.7873 | -0.6000 | 0.0261 | 0.5158 |
| IL1RN | -0.3714 | 0.1918 | 0.6277 | 0.1121 | 0.7043 | 0.9060 | -0.0989 | 0.7385 | 0.9136. | -0.2352 | 0.4175 | 0.7633 |
| D3 Cytokine Proteins | ||||||||||||
| IFN-α | -0.0632 | 0.8382 | 0.9484 | 0.1348 | 0.6589 | 0.8896 | 0.3026 | 0.3121 | 0.7212 | 0.2944 | 0.3264 | 0.7335 |
| IL-6 | 0.3232 | 0.2949 | 0.7046 | -0.0961 | 0.7564 | 0.9211 | 0.0874 | 0.7820 | 0.9263 | 0.3319 | 0.2820 | 0.6999 |
| IL-18 | -0.0660 | 0.8231 | 0.9451 | 0.2552 | 0.3757 | 0.7526 | 0.1694 | 0.5597 | 0.8284 | -0.1012 | 0.7298 | 0.9117 |
| IFN-γ | -0.3160 | 0.2689 | 0.6988 | -0.0022 | 0.9968 | 0.1000 | 0.3005 | 0.2870 | 0.7021 | -0.0354 | 0.9062 | 0.9557 |
| MCP-1 | -0.7890 | 0.0013 | 0.4228 | -0.2835 | 0.3253 | 0.7335 | 0.2527 | 0.3825 | 0.7548 | -0.5165 | 0.0615 | 0.5158 |
| IL-1RA | 0.0399 | 0.8967 | 0.9556 | -0.0446 | 0.8839 | 0.9547 | 0.1948 | 0.5030 | 0.7907 | 0.1338 | 0.6483 | 0.8824 |
bold font corresponds to p<0.05.
bold and italic font corresponds to p<0.01
Genes shared between the top five scoring pathways on D1 also included IL1RN, SOCS3, IFITM1, IRF7, NFKBIA, and KIT, genes that are included in the DEG.
The gene encoding IL-1RA is ILRN that is included in the DEG.