Figure 2.

Effects of IGF1R localization over the proliferation and migration of glioma cells. (A) Left: U87Empty (solid circles), U87WT (solid rhomboids), and U87Mut (solid triangles) cells were grown for 4 days with low serum concentration (1% FBS) or in the presence of 50 nM IGF1 [U87Empty + (open circles), U87WT+ (open rhomboids), and U87Mut+ (open triangles)]. The results of a representative experiment from a total of three performed in triplicates are shown; Right: total number of cells, according to cell line, days in culture, and treatment. Values are expressed as mean ± SD (*p < 0.05, ANOVA, Tukey’s post-test). (B) U87Empty, U87WT, and U87Mut cells were cultured 24 h in low-serum conditions (1% FBS) with or without 50 nM IGF1 stimulus, fixed, stained with PI, and analyzed by flow cytometry. A representative experiment is shown. (C) Wounding assays were performed to U87Empty, U87WT, and U87Mut cell confluent cultures. The cells were grown for 36 h in low-serum conditions (1% FBS) or in the presence of 50 nM IGF1. To test the specificity of the response, the cells were pre-incubated (1 h) with 0.5 μM OSI906 (OSI+IGF1). The results obtained from three independent experiments are presented as the proportion of covered area related to time 0. Values are expressed as mean ± SD (*p < 0.05, ANOVA, Tukey’s post-test).