FIGURE 4.

Effect of curcumin/cyclodextrin polymer (CUR/CDP) pretreatment on intracellular reactive oxygen species (ROS) in H₂O₂‐induced LO2 cells damage. LO2 cells were preincubated with CUR/CDP (10–40 µg/ml) for 12 h and then stimulated with H₂O₂ (700 µM) for additional 3 hr. 2′,7′ dichlorofluorescein (DCF) fluorescent density was detected by the ROS assay kit. p < 0.01 (**) means that columns between H₂O₂ group and CUR/CDP groups are significantly different. p < 0.01 (##) means that columns between control group and H₂O₂ group are significantly different