FIGURE 6.

Effects of curcumin/cyclodextrin polymer (CUR/CDP) on the expression levels of phosphorylated‐p53 and caspase‐3. The expression levels of phosphorylated‐p53 (a) and caspase‐3 (b) were detected by Western blotting analysis. LO2 cells were preincubated with CUR/CDP (10–40 µg/ml) for 12 h and then stimulated with H₂O₂ (700 µM) for additional 3 h. Protein expression level of phosphorylated‐p53 (c) and caspase‐3 (d) as percentage of control. p < 0.01 (**) means that columns between H₂O₂ group and CUR/CDP groups are significantly different. p < 0.01 (##) means that columns between control group and H₂O₂ group are significantly different