TABLE 2.
Serotype analysis of the RLPS
| RLPS source | Antiserum affinitya
|
|
|---|---|---|
| Anti-Rf205 | Anti-Rm41 | |
| S. fredii USDA191 | +++ | − |
| S. fredii USDA205 | +++ | − |
| Sinorhizobium sp. strain NGR234 | +++ | − |
| S. fredii USDA208 | ++ | − |
| S. meliloti AK631 | − | +++ |
| S. meliloti Rm1021 | − | +++ |
| S. meliloti NRG133 | − | +++ |
| S. fredii USDA192 | − | +++ |
| S. fredii USDA196 | − | +++ |
| S. fredii USDA197 | − | +++ |
| S. fredii USDA257 | − | +++ |
| S. meliloti NRG185 | − | ++ |
| S. meliloti NRG247 | − | ++ |
| S. meliloti NRG286 | − | ++ |
| S. fredii USDA201 | − | ++ |
| S. fredii HH103 | − | ++ |
| S. meliloti NRG23 | − | +/− |
| S. meliloti NRG53 | − | +/− |
| S. fredii HH303 | − | − |
| R. etli CE3 | − | − |
| B. japonicum USDA110 | − | − |
a
Polyclonal antisera included Anti-Rf205, raised against S. fredii USDA205, and anti-Rm41, raised against S. meliloti Rm41. Affinities were determined by immunoblot analysis (Fig. 4). +/−, weakly positive; ++, positive; +++, strongly positive; −, negative. R. etli CE3 and B. japonicum USDA110 were included as negative controls.