Table 1. Key Resources Table.
| Reagent or Resource | Sources | Identifier | Additional information |
|---|---|---|---|
| Antibodies | |||
| Anti phospho-Histone H3-rabbit | Cell signaling | 9701S | 1:200 v/v |
| Anti phospho-Histone H3-mouse | Cell signaling | 9706S | 1:200 v/v |
| Anti α-PheRS | Genescript | 4668 | Customized product (1:200 v/v) |
| Anti α-PheRS | Genescript | 4669 | Customized product (1:200 v/v) |
| Anti Myc-mouse | Developmental Studies Hybridoma Bank (DSHB) | 9E10 | Supernatant (1:3 v/v) |
| Anti Puromycin | DSHB | PMY-2A4 | 1:100 v/v |
| Anti Prospero | DSHB | MR1A | 1:200 v/v |
| Anti Delta | DSHB | C594.9B | 1:10 v/v |
| Anti V5 tag-rabbit | Cell signaling | 13202 | 1:200 v/v |
| Anti Cy3 rabbit | Jackson Immuno Research | 115-165-146 | 1:200 v/v |
| Anti-rabbit Alexa Flour 488 | Molecular Probes | A-11008 | 1:200 v/v |
| Anti-rabbit Alexa Flour 488 | Molecular Probes | A-11034 | 1:200 v/v |
| Anti-mouse Alexa Flour 488 | Molecular Probes | A-11029 | 1:200 v/v |
| Anti-rabbit Alexa Flour 488 | Life technology | A-21206 | 1:200 v/v |
| Anti-rabbit Alexa Flour 594 | Invitrogen | A-11037 | 1:200 v/v |
| Anti-mouse Alexa Flour 594 | Molecular Probes | A-11032 | 1:200 v/v |
| Anti-mouse Alexa Flour 568 | Life technology | A-10037 | 1:200 v/v |
| Anti α-tubulin | Abcam | Ab18251 | 1:1,000 v/v |
| Anti GFP | ImmunoKontact | 042704 | 1:1,000 v/v |
| Anti Myc-rabbit | Santa Cruz | Sc-789 | A-12 (1:1,000 v/v) |
| HRP anti-rabbit IgG antibody (Peroxidase) | Vector | PI-1000 | 1:10,000 v/v |
| HRP anti-rabbit IgG antibody (Peroxidase) | Vector | PI-2000 | 1:10,000 v/v |
| Fly stocks and genetics | |||
| α-PheRS G2060 /FM6 | Bloomington Drosophila Stock Center (BDSC) | 26625 | |
|
gα-PheRS
Cys
UAS-α-PheRS Cys |
Transgenic construct Transgenic construct |
||
| hspFLP; Act-Gal4/CyO; neoFRT82B, tub-Gal80/TM3, Sb | |||
| w; If/CyO; neoFRT82B, UAS-α-PheRS(Cys) | |||
| engrailed-Gal4 | BDSC | 30564 | |
| engrailed-Gal4, NRE-EGFP, UAS-myrRFP | BDSC | 30730 | |
| UAS-GFP | BDSC | 6658 | |
| w; UAS-Myc::MYC | BDSC | 9674 | |
| hspFLP/y; +; UAS-Myc::MYC | BDSC | 9675 | |
| hspFLP/y; UAS-NICD /CyO; MKRS/TM2 | BDSC | 52008 | |
| NRE-EGFP | BDSC | 30727 | |
| UAS-N RNAi | BDSC | 7078 | |
| neoFRT82B Sb1/TM6 | BDSC | 2051 | |
| NRE-eGFP | BDSC | 30728 | |
| UAS-Dl | BDSC | 5614 | |
| tub-Gal4/TM3, Sb | BDSC | 5138 | |
| w*, Insc-Gal4 | BDSC | 8751 | |
| w*, UAS-Dicer2; wor-Gal4, ase-Gal80; UAS-mCD8::GFP | IMBA | A gift from Juergen A. Knoblich, IMBA | |
| y w att2A[vas-ϕ]; +; attP-86F | ETH Zurich | A gift from Hugo Stocker, ETH | |
| esg-Gal4, UAS-2XEYFP; MKRS/TM6B, Tb | ETH Zurich | A gift from Hugo Stocker, ETH | |
| esg-Gal4, UAS-2XEYFP; tub-Gal80ts/TM3, Sb | ETH Zurich | A gift from Hugo Stocker, ETH | |
| yw; esg-Gal4, UAS-GFP/TM6B, Tb, Hu | ETH Zurich | 2400 | A gift from Hugo Stocker, ETH |
| NP1-Gal4 (Myo31DF or Myo1A-Gal4)/CyO, y+ | ETH Zurich | 2398 | A gift from Hugo Stocker, ETH |
| esg-Gal4, UAS-mCherry-CD8, tub-gal80ts/CyO | A gift from Péter Nagy, Cornell University | ||
| yw;UAS-cyto-gars-myc/CyO | A gift from Albena Jordanova, VIB-U Antwerp Center for Molecular Neurology | ||
| Bacteria strains and vectors | |||
| XL1 blue | Aligent | 200249 | |
| Rosetta–Novagen | Merck milipore | 70954 | |
| pET-28a –Novagen | Merck milipore | 69864 | |
| pET LIC (2A-T) | Addgene | 29665 | |
| pUASattB | Drosophila Genomics Resource Center | 1419 | |
| pw+SNattB | [79] | ||
| Commercial assay or kit | |||
| Pierce Silver Stain kit | Thermo Scientific | 24612 | |
| Pierce BCA Protein Assay kit | Thermo Scientific | 23227 | |
| ReliaPrep DNA CleanUp and Concentration System | Promega | A2893 | |
| GeneElute HP Plasmid miniprep kit | Sigma | NA0160 | |
| Qiagen Plasmid Plus Midi kit | Qiagen | 12943 | |
| Ni-NTA affinity resin | Qiagen | 30210 | |
| ECL Prime Western Blotting System | GE Healthcare | RPN2232 | |
| RNAMaxx High Yield Transcription Kit | Agilent | 200339 | |
| Software, algorithm | |||
| Leica Application Suite X (LAS X) | Leica | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ | |
| FIJI | ImageJ | https://fiji.sc/ | |
| GraphPad Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ | |
| FlowJo | BD Biosciences | https://www.flowjo.com/ | |
| Microsoft Excel | Microsoft | https://products.office.com/en-us/excel | |