Table 2. Buffers.

Lysis buffer for Drosophila tissue	Lysis buffer for bacteria
20 mM Tris HCl pH7.4 150uM NaCl 2 mM EDTA 50 mM NaF 10% Glycerol 1% Triton X100 1 Protease inhibitor cocktail tablet (Roche-4693159001) 1 mM phenylmethylsulphonyl fluoride	20 mM Tris HCl pH7.4 150uM NaCl 2 mM EDTA 50 mM NaF 10% Glycerol 1% Triton X100 4mM Imidazole 1M 0.6% Lysozyme 1 Protease inhibitor cocktail tablet 1 mM phenylmethylsulphonyl fluoride
4% PFA	1X PBST
1X PBST 4% (w/v) Paraformaldehyde	0.2% (v/v) Tween 20 1X PBS
Blocking buffer	Fly food recipe
5% (w/v) non-fat dry milk 0.1% (v/v) Triton X100	20.4 l H20 1,680 g Maize flour 720 g Yeast 1,800 g Syrup 192 g Potassium sodium tartrate tetrahydrate 36 g Nipagin 120 ml Propionic acid
10X PBS pH 7.4
10.6 mM KH2PO4 1.5 M NaCl 30 mM Na2PO4.7H20
10X SDS running buffer	10X Transfer buffer
30 g Tris base 144 g Glycine 10 g SDS dH2O to 1 L	30 g Tris base 144 g Glycine dH2O to 1 L
10X TBS pH to 7.6	1X TBST
24 g of Tris Base 88 g of NaCl dH2O to 1 L	100 mL 10X TBS 900 mL dH2O 0.1% (v/v) Tween 20