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. 2022 May 11;8(19):eabm5371. doi: 10.1126/sciadv.abm5371

Fig. 1. PR8-miR133b/206 is attenuated in myoblast cells in vitro.

Fig. 1.

(A) Schematic of miRNA-targeting strategy. Target sequences of two miRNAs expressed in cardiac cells, miR133b and miR206, or a length-matched random sequence were inserted into the influenza A PR8 NP gene, along with a duplicated NP packaging sequence, to generate replication-competent virus with heart-specific attenuation (PR8-miR133b/206) or control virus (PR8-miRctrl). (B) C2C12 cells or (C) HEK-293T cells were infected with PR8-miR133b/206 or PR8-miRctrl for 24 hours at a multiplicity of infection of 2.5, and percent infection was determined by flow cytometry. Graphs represent normalized infection values. *P < 0.05 by unpaired t test; NS, not significant.