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. 2022 May 11;8(19):eabn1805. doi: 10.1126/sciadv.abn1805

Fig. 7. Improved antitumor efficacy of Mixture via two PTT treatments (1 and 4 hours), assisted by aPD-L1.

Fig. 7.

(A) Schematic illustration of antitumor experiment in mice by Mixture with two NIR laser irradiations (808 nm for 5 min each time) at 1 and 4 hours, respectively, after administration, assisted by immune checkpoint blockage. (B) The change of tumor volume in mice treated with PBS, M-GNPs (1 hour), Mixture (1 hour), Mixture (1 and 4 hours), and Mixture (1 and 4 hours) + aPD-L1 at a GNP dose of 10 mg/kg and 8 μg of aPD-L1 per mouse every 3 days for five doses in total (n = 6). (C) Photographs of the tumor tissues collected on day 7. I: PBS. II: M-GNPs (1 hour). III: Mixture (1 hour). VI: Mixture (1 and 4 hours). V: Mixture (1 and 4 hours) + aPD-L1. (D) Photographs of the lung tissues collected on day 7 and quantitative analysis on metastatic nodules. (E) H&E staining, TUNEL staining, and caspase-3 staining of the tumor tissues collected on day 7. Scale bar, 100 μm. The experiments for (C), (D), and (E) were repeated three times (n = 3) and data were presented as means ± SD. All statistical analyses were performed using one-way ANOVA. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.