Fig. 9. Disruption of dME3 impairs the biogenesis of CI AIs.
Mitochondrial preparations from flight muscles isolated from Dmef2-Gal4/w1118 (wild-type) and Dmef2-Gal4/UAS-dME3-RNAi flies 2 days after eclosure were analyzed by BN-PAGE, followed by immunoblotting with the antibodies listed. The antibodies used were anti-NDUFS3 (A), anti-dNDUFA9 (B), anti-dNDUFV3 (C) anti-dNDUFV1 (D), anti-dNDUFS5 (E), anti-dNDUFA11 (F), anti-dND3 (G), dNDUFB5 (H), and anti-dND5 (I). Anti–ATP synthase, subunit B was used as a loading control. AIs denoted with # and * were quantified. See figs. S16 to S18 for replicates and quantification of immunoblots.