. 2022 Apr 27;13:875887. doi: 10.3389/fphar.2022.875887

TABLE 3.

Lymphoid populations (absolute counts) in the lung of SP-C^I73TGATA1^WT and SP-C^I73TGATA1^KO mice.

Lymphoid	Genotype	CTL (n=6)	7d (n=5)	14d (n=4)	42d (n=4)
Total Lymphocytes CD3⁺	SPC^I73TGATA1^WT	345,631 ± 86,296	183,554 ± 39,269	395,329 ± 56,594	190,527 ± 65,449
Total Lymphocytes CD3⁺	SPC^I73TGATA1^KO	250,269 ± 79,960	360,842 ± 47,500	589,159 ± 253,538	126,307 ± 25,065 ^c
T Cells CD3⁺CD4⁺	SPC^I73TGATA1^WT	195,017 ± 54,159	69,888 ± 14,055	143,506 ± 17,513	82,764 ± 28,488
T Cells CD3⁺CD4⁺	SPC^I73TGATA1^KO	96,382 ± 29,041	160,060 ± 22,763	261,151 ± 119,068	64,875 ± 12,762
T Cells CD3⁺CD8⁺	SPC^I73TGATA1^WT	56,669 ± 14,499	42,242 ± 10,950	89,201 ± 7,790	34,887 ± 12,703
T Cells CD3⁺CD8⁺	SPC^I73TGATA1^KO	81,096 ± 27,228	75,407 ± 1,942	146,561 ± 78,064	18,951 ± 4,028 ^c
Dendritic Cells CD4⁺CD103⁺	SPC^I73TGATA1^WT	5,220 ± 1,415	4,584 ± 1,254	61,332 ± 22,982 ^a ^, ^b	6,324 ± 2,890 ^c
Dendritic Cells CD4⁺CD103⁺	SPC^I73TGATA1^KO	1,894 ± 549	20,495 ± 10,423	25,963 ± 8,265	4,703 ± 722
Dendritic Cells CD8⁺CD103⁺	SPC^I73TGATA1^WT	63,210 ± 14,376	46,197 ± 13,312	24,085 ± 2,449	42,933 ± 14,522
Dendritic Cells CD8⁺CD103⁺	SPC^I73TGATA1^KO	39,530 ± 15,995	66,423 ± 7,023	55,100 ± 30,155	20,219 ± 4,546
Total B Cells B220⁺	SPC^I73TGATA1^WT	355,729 ± 116,677	121,006 ± 36,745	174,755 ± 42,299	185,453 ± 74,308
Total B Cells B220⁺	SPC^I73TGATA1^KO	575,678 ± 176,291	277,739 ± 66,428	380,566 ± 195,524	79,770 ± 17,796 ^a
B Cells B220⁺CX3CR1^-	SPC^I73TGATA1^WT	136,526 ± 50,823	34,278 ± 12,104	105,394 ± 18,429	79,686 ± 33,316
B Cells B220⁺CX3CR1^-	SPC^I73TGATA1^KO	161,705 ± 47,002	105,698 ± 40,083	75,482 ± 42,431	10,523 ± 2,139 ^a
B Cells B220⁺CX3CR1⁺	SPC^I73TGATA1^WT	266,498 ± 75,310	86,729 ± 24,875	69,362 ± 26,868	105,766 ± 41,078
B Cells B220⁺CX3CR1⁺	SPC^I73TGATA1^KO	413,972 ± 129,402	172,040 ± 27,728	262,333 ± 170,944	69,247 ± 15,758 ^a

Single cell suspensions of SP-C^I73TGATA1^WT and SP-C^I73TGATA1^KO lung digests was assessed by flow cytometry, enriched in viable CD45⁺ singlets, and analyzed following gating strategy described in the Methods section. Absolute counts are presented as means ± SEM (N=4-6 mice/condition). Data were analyzed by two-way ANOVA, with Tukey post-hoc test.

^aSignificant compared to controls (CTL).

^bSignificant compared to 7 d group.

^cSignificant compared to 14 d group.